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GrainGenes Reference Report: ASI-30-855

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Reference	ASI-30-855
Title	Tissue culture response and selection of transgenic receptors in wheat ( Triticum aestivum L.) cultivars
Journal	Acta Agronomica Sinica
Year	2004
Volume	30
Pages	855-860
Author	Ji Q Ren Z
Abstract	In the study on transferring alien genes into wheat using a transgenic system, the receptor plant is very important for its capability in callus induction and plant regeneration. The present study was conducted to determine the good germplasm in wheat breeding for transgenic receptors, which exhibit excellence in tissue culture response and agronomic characteristics. A total of 75 local genotypes, 83 modern genotypes and 127 F SUB 5 families of Douhuabai/R1395 were analysed for days of callusing at 50% (DCH), rate of callusing in four weeks (RCW) in callus induction; rate of callusing in root formation (RCRF) and rate of callusing in shoot formation (RCSF) in differentiation culture. An extensive variation was found in DCH, RCW, RCRF and RCSF among different wheat genotypes. A high correlation between DCH and RCW was found with the correlation coefficients of -0.7229**, indicating that there was same genetic background for DCH and RCW. No significant correlation was observed among DCH, RCRF and RCSF, suggesting that the characters were controlled by different genetic foundations. A moderate correlation was observed between DCH and RCW with some agronomic characters (plant height, number of seeds per spike, grain weight per spike and 1000-grain weight), indicating the possibility to develop wheat transgenic receptors with excellent agronomic characteristics. Several excellent parents of wheat with high ability in tissue culture response, such as Douhuabai from Tibet and R1395 from advanced lines, were screened from the genotypes. A higher frequency of lines with excellent ability in tissue culture response was observed in the F SUB 5 family populations of the crosses between Douhuabai and R1395 as well as other wheat parents with excellent agronomic traits. The method of wheat breeding for transgenic receptors is discussed
Keyword	[ Hide all but 1 of 20 ] agronomic characteristics callus characteristics correlation analysis cross cultivar differentiation genetic transformation genotype germplasm in vitro culture plant breeding methods plant height regeneration root seed seed weight selection tissue culture transgenic plants

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