GrainGenes Reference Report: MPM-17-1078
Reference
MPM-17-1078
Title
Nitrogen fixation in wheat provided by Klebsiella pneumoniae 342
Journal
Molecular Plant-Microbe Interactions
Year
2004
Volume
17
Pages
1078-1085
Author
Iniguez AL Dong YM Triplett EW
Abstract
In this report, all of the criteria necessary for the demonstration of nitrogen fixation in wheat (Triticum aestivum L.), the world's most important crop, are shown upon inoculation with a nitrogen-fixing bacterium, Klebsiella pneumoniae 342 (Kp342). Kp342 relieved nitrogen (N) deficiency symptoms and increased total N and N concentration in the plant. Nitrogen fixation was confirmed by 15N isotope dilution in the plant tissue and in a plant product, chlorophyll. All of these observations were in contrast to uninoculated plants, plants inoculated with a nitrogen-fixing mutant of Kp342, and plants inoculated with dead Kp342 cells. Nitrogenase reductase was produced by Kp342 in the intercellular space of the root cortex. Wild-type Kp342 and the nifH mutant colonized the interior of wheat roots in equal numbers on a fresh weight basis. The nitrogen fixation phenotype described here was specific to cv. Trenton. Inoculation of cvs. Russ or Stoa with Kp342 resulted in no relief of nitrogen deficiency symptoms
External Databases
Pubmed: 15497400
Keyword
[ Hide all but 1 of 14 ] chlorophyll cortex cultivar mutant nitrogen nitrogen fixation nitrogen fixing bacteria nitrogenase nitrogenfixing bacteria nutrient deficiencies phenotype plant nutrition root symptoms
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GrainGenes Reference Report: MPM-17-1078
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||