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GrainGenes Reference Report: MPM-15-445

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Reference	MPM-15-445
Title	Functional and comparative bioinformatic analysis of expressed genes from wheat spikes infected with Fusarium graminearum
Journal	Molecular Plant-Microbe Interactions
Year	2002
Volume	15
Pages	445-455
Author	Kruger WM Pritsch C Chao SM Muehlbauer GJ
Abstract	Summary: Fusarium head blight, caused by the fungus Fusarium graminearum, is a major disease on wheat (Triticum aestivum L.). Expressed sequence tags (ESTs) were used to identify genes expressed during the wheat-F graminearum interaction. We generated 4,838 ESTs from a cDNA library prepared from spikes of the partially resistant cultivar Sumai 3 infected with F. graminearum. These ESTs were composed of 2,831 singlet (single-copy transcripts) and 715 contigs (multiple-copy transcripts) for a total of 3,546 nonredundant sequences. Four sets of nonredundant sequences were identified. One set contains numerous, common biotic and abiotic stress-related genes. Many of these stress-related genes were represented by multiple ESTs, indicating that they are abundantly expressed. A second set comprised 16 nonredundant sequences from F. graminearum that may be required for pathogenicity. A subset of these fungal genes encodes proteins associated with plant cell wall degradation. A third set of 326 nonredundant sequences had no DNA or amino acid sequence similarity to almost 1 million plant and over 7 million animal sequences in dbEST (as of 22 June 2001). Thus, these 326 nonredundant sequences have only been found in our F. graminearum-infected 'Sumai 3' cDNA library. A fourth set of 29 nonredundant sequences was found in our F. graminearum-infected wheat and another plant-pathogen interaction cDNA library. Some of these sequences encode proteins that may act in establishing various plant-fungal interactions
External Databases	Pubmed: 12036275
Keyword	[ Hide all but 1 of 45 ] amino acid amino acid sequence blight carbohydrate-binding cdna cdna library cell contig culmorum cultivar defense response genes degradation disease disease resistance dna expressed sequence tags fungal fungus fungus fusarium fusarium fusarium graminearum fusarium head blight gene genomics gibberella zeae graminearum head blight messenger rna multiple pathogenicity plant cell wall plant disease-resistance plant pathogen reduced virulence resistant sequence sequence similarities sequence tags similarities single copy spike stress-related genes triticum triticum aestivum wall

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