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GrainGenes Reference Report: MPM-14-358

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Reference	MPM-14-358
Title	Comparison of benefit to sugarcane plant growth and N-15(2) incorporation following inoculation of sterile plants with Acetobacter diazotrophicus wild-type and Nif(-) mutant strains
Journal	Molecular Plant-Microbe Interactions
Year	2001
Volume	14
Pages	358-366
Author	Sevilla M Burris RH Gunapala N Kennedy C
Abstract	Summary: The ability of the nitrogen-fixing bacterial endophyte Acetobacter diazotrophicus strain PA15 to enhance the growth of sugarcane SP70-1143 was evaluated in the growth chamber, greenhouse, and field by comparing plants inoculated with wild-type and Nif- mutant MAd3A in two independent experiments. The wild-type and Nif- mutant strains colonized sugarcane plants equally and persisted in mature plants. In N-deficient conditions, sugarcane plants inoculated with A. diazotrophicus PA15 generally grew better and had a higher total N content 60 days after planting than did plants inoculated with mutant Mad3A or uninoculated plants. These results indicate that the transfer of fixed N from A. diazotrophicus to sugarcane might be a significant mechanism for plant growth promotion in this association. When N was not limiting, growth enhancement was observed in plants inoculated with either wild-type or Nif- mutants, suggesting the additional effect of a plant growth promoting factor provided by A. diazotrophicus. A 15N2 incorporation experiment demonstrated that A. diazotrophicus wild-type strains actively fixed N2 inside sugarcane plants, whereas the Nif- mutants did not
Keyword	[ Hide all but 1 of 18 ] acetobacteraceae azospirillum biological nitrogen fixation cane colonization culture diazotrophs endophyte growth infection inoculation mechanism meristem tissue culture mutant nitrogen-fixation seedlings sugarcane transfer

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