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GrainGenes Reference Report: MGG-262-846

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Reference	MGG-262-846
Title	Genetic and physical characterization of grain texture-related loci in diploid wheat
Journal	Molecular and General Genetics
Year	1999
Volume	262
Pages	846-850
Author	Tranquilli G Lijavetzky D Muzzi G Dubcovsky J
Abstract	Endosperm texture, i.e. the hardness or softness of the grain, is an important quality criterion in cereals because it determines many grain end-use properties. Grain softness is the dominant trait and is mainly controlled by the Ha locus on the short arm of chromosome 5D in hexaploid bread wheat. Genes for puroindoline a (Pina-D1), puroindoline b (Pinb-D1), and grain softness related protein (Gsp-D1) have been shown to be linked to the Ha locus in different mapping populations and have been associated with the expression of grain softness. The study of the linkage relationships among these genes has been limited by the low level of polymorphism in the D genome of hexaploid Triticum aestivum. In the present study, a highly polymorphic Triticum monococcum mapping population was used to analyze linkage relationships among these three genes. Gsp-A m 1 and Pina-A m 1 were found to be completely linked and lie 0.14 cM distal to Pinb-A m 1 in the distal region of the short arm of chromosome 5Am. The tight genetic linkage among these three genes was paralleled by their physical proximity within a single 105-kb clone isolated from a T. monococcum bacterial artificial chromosome (BAC) library. A restriction map of this BAC clone showed that Pina-A m 1 is located between Pinb-A m 1 and Gsp-A m 1. Partial sequences of the T. monococcum genes showed a high degree of similarity with their T. aestivum counterparts (>= 94%). Marker-assisted selection strategies based on the tight linkage among Ha-related genes are discussed.
External Databases	Pubmed: 10628869 https://doi.org/10.1007/s004380051149
Gene	Pina-A^m^1 (Triticum) Pinb-A^m^1 (Triticum)
Germplasm	T. monococcum ssp. monococcum DV92 T. monococcum ssp. aegilopoides G3116
Keyword	[ Hide all but 1 of 47 ] artificial chromosome bac base sequence bread bread wheat d genome diploidy dna dna primers endosperm endosperm texture gene genetic linkage genetic markers genome germany hard seeds hardness hexaploid library linkage linkage groups loci locus map mapping marker marker assisted selection molecular mapping physical chromosome mapping polymorphism polymorphism,restriction fragment length population puroindoline puroindoline-a puroindoline-b quality quantitative traits restriction mapping rflp selection sequence softness texture triticum triticum aestivum triticum monococcum

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