Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: MGG-241-674

[Submit comment/correction]

Reference	MGG-241-674
Title	Anther culture and Hordeum bulbosum-derived barley double haploids: mutations and methylation
Journal	Molecular and General Genetics
Year	1993
Volume	241
Pages	674-679
Author	Devaux P Kilian A Kleinhofs A
Abstract	Anther culture and Hordeum bulbosum-derived doubled haploid (DH) lines of barley (Hordeum vulgare L. were analyzed for RFLP and RAPD polymorphisms Polymorphisms were not detected in the anther culture- or H bulbosum-derived DH lines among 273 RFLP and 89 polymerase chain reaction (PCR)-amplified DNA fragments assayed It was calculated that base substitution or small deletion/insertion mutations had not been induced among 401 640 bp screened Large deletion/insertion mutations were not observed among 33 Mb screened Polymorphisms were observed when DNA was digested with the methylation-sensitive restriction enzymes HpaII and MspI: these RFLPs originated primarily from the anther culture-derived doubled haploids. The data indicate that heritable DNA methylation changes had occurred during DH production, particularly with the anther culture method.
Keyword	[ Hide all but 1 of 13 ] anther-culture dna methylation genetic markers genetic polymorphism haploid hordeum bulbosum hordeum vulgare lines mutation plant breeding plant breeding-methods random-amplified-polymorphic-dna restriction fragment length polymorphism

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.