GrainGenes Reference Report: MGG-229-389
Reference
MGG-229-389
Title
Nucleotide sequence and molecular analysis of the low temperature induced cereal gene BLT4.
Journal
Molecular and General Genetics
Year
1991
Volume
229
Pages
389-394
Author
Dunn MA
[ Show all 6 ]
Abstract
The nucleotide sequence and derived amino acid sequence of a cDNA clone (BLT4) for a low temperature induced barley gene were determined. This gene, together with a small family of related genes, was shown to reside on chromosome 3. The BLT4 clones has homology with genes in wheat and oats. Its expression was studied in oats and in barley doubled haploid lines segregating for spring/winter habit and for frost hardiness. These analyses show that elevated steady state levels of BLT4 mRNA are produced in shoot meristematic tissue after 3 days low positive temperature treatment. The low temperature response was found in all barley doubled haploid lines and was therefore not associated specifically with either the spring/winter habit or frost hardiness. Elevated levels of BLT4 mRNA were also seen in drought-stressed barley and it is likely that this is a gene encoding a low molecular weight protein that is responsive to dehydrative stresses, such as cold and drought.
Gene
Cig7 (Hordeum) blt4 (Hordeum)
Probe
pBLT4
Keyword
[ Hide all but 1 of 11 ] amino acid sequence Barley drought frost hardiness gene expression gene homology molecular sequence data oats spring-winter habit stress wheat
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GrainGenes Reference Report: MGG-229-389
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||