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GrainGenes Reference Report: MBR-12-51

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Reference	MBR-12-51
Title	Gene-assisted selection for high molecular weight glutenin subunits in wheat doubled haploid breeding programs
Journal	Molecular Breeding
Year	2003
Volume	12
Pages	51-59
Author	Radovanovic N Cloutier S
Abstract	Summary: Molecular markers based on DNA sequence variations of the coding and/or promoter regions of the wheat (Triticum aestivum L.) HMW glutenin genes located at the Glu-1 loci were developed. Markers characteristic of alleles Glu-A1-1a (encoding Ax1 subunit) and Glu-A1-1c (encoding Ax2* subunit) at the Glu-A1 locus, alleles Glu-B1ak (encoding Bx7* subunit) and Glu-B1al for overexpressed Bx7 subunit at the Glu-B1 locus and alleles Glu-D1-1a (encoding Dx2 subunit) and Glu-D1-1d (encoding Dx5 subunit) at the Glu-D1 locus were tested using genomic DNA of haploid leaf tissue. A method for simultaneously extracting DNA from 96 haploid leaf tissue pieces is described. Two of the developed markers were dominant and two were co-dominant. A F1-derived population segregating for all HMW glutenin genes was used to test the validity of the markers and their usefulness in doubled haploid breeding programs. SDS-PAGE analysis of seed storage protein was performed on seeds from the doubled haploid lines. A total of 299 lines were tested with the DNA markers on the haploid tissue and validated by protein analysis of the corresponding DH seeds. PCR markers and SDS-PAGE analysis showed between 2 and 8.5% discrepancies depending on the marker. Applications of DNA markers for gene-assisted-selection of haploid tissue and use in breeding programs are discussed. Advantages and disadvantages of dominant and co-dominant markers are outlined
Keyword	[ Hide all but 1 of 47 ] bread wheat breeding breeding program codominant dna doubled haploid doubled haploid breeding gene gene-assisted selection genomic genomic dna glu-1 glu-a1 glu-b1 glu-d1 locus glutenin glutenin gene haploid haploid breeding high molecular weight high molecular weight glutenin subunits hmw glutenin hmw glutenin genes leaf tissue molecular weight pcr pcr analysis pcr marker population promoter promoter regions protein protein analysis sds-page analysis seed seed storage protein selection sequence sequence variation storage storage protein subunit tissue triticum triticum aestivum weight glutenin subunits x-type

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