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GrainGenes Reference Report: MBR-8-273

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Reference	MBR-8-273
Title	Transgenic rice with reduced glutelin content by transformation with glutelin A antisense gene
Journal	Molecular Breeding
Year	2002
Volume	8
Pages	273-284
Author	Maruta Y Ueki J Saito H Nitta N Imaseki H
Abstract	Summary: Rice plants were genetically transformed with a glutelin A antisense gene construct that was characterized by eight tandem repeats of a ca. 0.3 kb fragment at the 5' end of full-length glutelin A cDNA. Two transgenic rice lines, H39-59 and H75-3, homozygous for the glutelin A antisense gene were selected from T2 plants, and their T4 generation was examined. When grown in an open paddy field, T4 plants of both transgenic lines showed 20% to 40% reduction in the glutelin content of seeds and an increase in the prolamine content. The total seed-protein content of the two transgenic lines was not significantly different from that of the original variety, Tsukinohikari. Northern blot analysis revealed that the steady-state levels of glutelin A transcript also decreased and those of prolamine transcript increased in both H39-59 and H75-3 transgenic lines, and the changes in glutelin mRNA corresponded to changes observed in glutelins during seed development. Rice wine, sake, brewed from grains of the transgenic lines was found to contain 20% to 40% less amino acids and was evaluated as superior in quality to that made from the non-transgenic rice grains
Keyword	[ Hide all but 1 of 62 ] acid agrobacterium amino acids analysis antisense antisense dna antisense gene brewing quality cdna development dna endosperm field food quality gene gene expression gene transfer generation genes genetic transformation glutelin glutelin content grain grains homozygous kb level lines messenger rna messenger rnas mrna mutant northern northern blot nucleotide sequence oryza sativa paddy field protein content quality reduction rice rice plant rice plants rice wine sake seed seed development seed protein seeds steady-state levels storage protein storage protein glutelin tandem tandem repeat tobacco transcript transformation transgenic transgenic plant transgenic plants transgenic rice varieties

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