Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: MBR-8-103

[Submit comment/correction]

Reference	MBR-8-103
Title	Analysis of dough functionality of flours from transgenic wheat
Journal	Molecular Breeding
Year	2001
Volume	8
Pages	103-108
Author	Alvarez ML Gomez M Carrillo JM Vallejos RH
Abstract	Summary: The rheological properties of flours from five different lines of transgenic wheat that either express or over-express subunits 1Ax1 and 1Dx5 were analyzed by mixograph assays and SDS sedimentation tests. In one case, the over-expression of subunit 1Dx5 resulted in a ca. 2-fold increase in mixing time, associated with a significant improvement in dough strength, and a lower resistance breakdown, suggesting an important increase in dough stability. However, the flour failed to develop properly without mixing with control flour because the rate of mixing was insufficient to develop the dough, i.e., the flour was overstrong. In two wheat transgenic lines, the expression of 1Ax1 and 1Dx5 transgenes, associated with silencing of all the endogenous high-molecular-weight glutenin subunits, resulted in flours with lower mixing time, peak resistance and sedimentation volumes, suggesting a lower gluten strength
Keyword	[ Hide all but 1 of 40 ] allelic variation assay breadmaking breadmaking quality dough flour functionality gluten gluten strength glutenin glutenin subunit high molecular weight high molecular weight glutenin subunit hmw glutenin subunits improvement increase mixing mixing time mixograph molecular-weight subunits over-expression overexpression properties protein rate resistance rheological properties sds sds sedimentation sds sedimentation test sedimentation sedimentation test sedimentation volume stability subunit transformation transgene transgenic transgenic wheat volume

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.