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GrainGenes Reference Report: MBR-7-317

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Reference	MBR-7-317
Title	Tobacco matrix attachment region sequence increased transgene expression levels in rice plants
Journal	Molecular Breeding
Year	2001
Pages	317-327
Author	Cheng Z Targolli J Wu R
Abstract	Summary: In this study, six plasmids were constructed to study the effects of the tobacco Rb7 matrix attachment region (MAR) sequence on rice transgene expression. Among them, each of four plasmids contained two identical copies of the MAR sequence flanking two different reporter genes, which encode the green fluorescent protein and beta-glucuronidase. Two control plasmids contained no MAR sequences. Microprojectile bombardment was used to separately introduce these six plasmids into rice calli. Transgenic rice plants were regenerated, and gene expression was measured in rice leaf extracts of two-month-old transgenic plants. By comparing transgenic plants with the corresponding control plants, transgenic plants harboring each of four plasmids that included the MAR sequence resulted in average gene expression levels enhanced by 3.3-fold, 18-fold, 376-fold and 650-fold. These results suggest that the same MAR sequence can affect the expression of different genes to different extents. One goal of plant scientists and breeders is to maximize expression levels of transgenes, especially in the production of transgene-encoded protein. Therefore, inclusion of the Rb7 MAR sequence would be beneficial
Keyword	[ Hide all but 1 of 42 ] actin animal proteins attachment beta-glucuronidase biolistics bombardment calli copies encode gene gene expression genetic transformation green fluorescent protein histoenzymology jellyfish matrix matrix attachment region microprojectile bombardment nicotiana tabacum nucleotide sequence oryza sativa plasmid plasmid vectors plasmids production promoters protein recombinant dna region reporter reporter gene rice rice leaves rice plant sequence tobacco transgene transgene expression transgenic transgenic plant transgenic rice transgenic rice plants

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