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GrainGenes Reference Report: MBR-7-301

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Reference	MBR-7-301
Title	The expression of a maize glutathione S-transferase gene in transgenic wheat confers herbicide tolerance, both in planta and in vitro
Journal	Molecular Breeding
Year	2001
Pages	301-315
Author	Milligan AS Daly A Parry MAJ Lazzeri PA Jepson I
Abstract	Summary: Maize (Zea mays), in common with a number of other important crop species, has several glutathione S-transferase (GST) isoforms that have been implicated in the detoxification of xenobiotics via glutathione conjugation. A cDNA encoding the maize GST subunit GST-27, under the control of a strong constitutive promoter, was introduced into explants of the wheat (Triticum aestivum L.) lines cv. Florida and L88-31 via particle bombardment, using the phosphinothricin acetyltransferase (pat) gene as a selectable marker. All six independent transgenic wheat lines recovered expressed the GST-27 gene. T1 progeny of these wheat lines were germinated on solid medium containing the chloroacetanilide herbicide alachlor, and tolerance to this herbicide was correlated with GST-27 expression levels. In glasshouse sprays, homozygous T2 plants were resistant not only to alachlor but also to the chloroacetanilide herbicide dimethenamid and the thiocarbamate herbicide EPTC. These additional GST-27 activities, demonstrated via over-expression in a heterologous host, have not been described previously. T2 plants showed no enhanced tolerance to the herbicides atrazine (an s-triazine) or oxyfluorfen (a diphenyl ether). In further experiments, T2 wheat plants were recovered from immature transgenic scutella cultured on medium containing 100 mg/l alachlor, a concentration which killed null segregant and wild-type scutella. These data indicate the potential of the maize GST-27 gene as a selectable marker in wheat transformation
Keyword	[ Hide all but 1 of 69 ] acetyltransferase aestivum alachlor atrazine biolistics bombardment cdna chloroacetanilide herbicides common complementary dna conjugation crop crop species detoxification eptc explants expression florida gene gene transfer genes glasshouse glutathione glutathione conjugation glutathione transferase glutathione-s-transferase herbicide herbicide resistance herbicide tolerance herbicides homozygous host in vitro in vitro selection isoform level line maize marker marker genes media overexpression oxyfluorfen particle bombardment phosphinothricin progeny promoter regenerative ability resistant s-transferase scutellum selectable marker species subunit tolerance transformation transgenic transgenic plants transgenic wheat triticum triticum aestivum vitro wheat wheat plants wheat transformation wild-type xenobiotics zea zea mays

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