Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: MBR-5-579

[Submit comment/correction]

Reference	MBR-5-579
Title	PCR detection of genetically modified soya and maize in food stuffs
Journal	Molecular Breeding
Year	1999
Volume	5
Pages	579-586
Author	Hurst CD Knight A Bruce IJ
Abstract	Summary: The detection of genetically modified foodstuffs is becoming both a food sales and legal necessity. This study reports a rapid DNA extraction/PCR-based method for the detection of genetically modified soya (GMS) and maize (GMM) in mixed samples of transgenic and unmodified soybeans and maize kernels, and a variety of processed samples including soya flour, soya protein isolates, extruded defatted soya, acid- and alcohol-precipitated soya concentrates, soya lecithin, maize grits, seasoned corn puffs and salted corn chips. The presence of GMS DNA was determined with two pairs of primers directed towards different GMS target sequences and GMM by one primer pair. In addition, a multiplex PCR reaction which utilises an internal positive control was developed for both genetically modified organisms (GMOs). Results indicated that the methods are sensitive and specific enough to detect GMS down to a level of 0.01% dry weight in single-product PCRs and 0.1% in multiplex PCRs and GMM down to 0.001% dry weight in single-product PCRs and 0.01% in multiplex PCR. The methods are considered to represent a viable route for the commercial detection of GMS and GMM in foodstuffs
Keyword	[ Hide all but 1 of 31 ] alkyl (aryl) transferases bacillus thuringiensis cereal products concentrates corn detection dna dry weight endotoxins flour food foods genetic engineering genetic lly modified organism glycine max glyphosate gmo herbicide resistance isolate kernels maize multiplex pcr pcr polymerase chain reaction primer sequence soybeans transgenic transgenic plant weight zea mays

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.