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GrainGenes Reference Report: JPY-162-81

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Reference	JPY-162-81
Title	Asparagine synthetase gene TaASN1 from wheat is up-regulated by salt stress, osmotic stress and ABA
Journal	Journal of Plant Physiology
Year	2005
Volume	162
Pages	81-89
Author	Wang H Liu D Sun J Zhang A
Abstract	Differences in gene expression between salinity stressed and normally grown wheat seedlings were compared by the differential display (DD) technique. One DD-derived cDNA clone was characterized as a partial sequence of the wheat asparagine synthetase (AS) gene by sequence analysis and homology search of GenBank databases. Two AS genes of wheat, TaASN1 and TaASN2 , were further isolated by the RT-PCR approach. Comparison of the deduced polypeptide of TaASN1 and TaASN2 with AS proteins from other organisms revealed several homologous regions, in particular, the conserved glutamine binding sites and Class-II Glutamine amidotransferases domain. The functionality of TaASN1 was demonstrated by complementing an Escherichia coli asparagine auxotroph. TaASN1 transcripts were detected in roots, shoots, anthers and young spikes by RT-PCR analysis. Abundance of TaASN1 mRNA in young spikes and anthers was higher than that in shoots and roots under normal growth conditions. TaASN1 was dramatically induced by salinity, osmotic stress and exogenous abscisic acid (ABA) in wheat seedlings. TaASN2 transcripts were very low in all detected tissues and conditions and were only slightly induced by ABA in roots
External Databases	Pubmed: 15700423
Keyword	[ Hide all but 1 of 32 ] abscisic acid anthers asparagine aspartate-ammonia ligase binding sites biology cdna clone complementary dna databases dna cloning enzymes gene gene expression genes genetics glutamine growth messenger rna nucleotide sequences osmosis protein proteins root roots salinity salt stress seedlings shoots spikes stress stress response wheat

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