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GrainGenes Reference Report: JPY-158-1601

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Reference	JPY-158-1601
Title	Light and sugar regulation of the barley sucrose: fructan 6-fructosyltransferase promoter
Journal	Journal of Plant Physiology
Year	2001
Volume	158
Pages	1601-1607
Author	Nagaraj VJ Riedl R Boller T Wiemken A Meyer AD
Abstract	Summary: Fructans are a class of major storage compounds in many plants including several economically important cereals. Earlier we characterized and cloned the sucrose: fructan 6-fructosyltransferase (6-SFT) of barley (Hordeum vulgare L.), a key enzyme for the synthesis of branched fructans (graminans) typical of many cereals and temperate forage grasses. Here, we describe the cloning of the barley 6-SFT promoter region, by PCR-based genome walking procedures. Using a promoter-reporter gene construct (uidA encoding beta-glucuronidase) and microprojectile bombardment of excised barley leaves, we show that the cloned sequence contains the necessary cis acting elements conferring sucrose and light induction of 6-SFT transcription
Keyword	[ Hide all but 1 of 44 ] 1-fructosyltransferase 6-fructosyltransferase 6-sft barley barley leaves bombardment carbohydrate metabolism cereal cloning contains element enzyme expression forage forage grasses fructan gene genome grass hordeum hordeum vulgare identification induction key enzyme l-cv gerbel leaf light light induction light-regulation metabolism microprojectile bombardment promoter region regulation sequence storage sucrose sucrose : fructan 6-fructosyltransferase sugar sugar-sensing synthesis transcription uida vulgare

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