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GrainGenes Reference Report: JPY-158-1541

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Reference	JPY-158-1541
Title	The transcripts of several components of the protein synthesis machinery are cold-regulated in a chloroplast-dependent manner in barley and wheat
Journal	Journal of Plant Physiology
Year	2001
Volume	158
Pages	1541-1546
Author	Baldi P [ Show all 7 ]
Abstract	Summary: Nine clones coding different components of the protein synthesis machinery were selected from an EST barley library prepared from cold exposed plants and tested for their expression at low temperature. Northern analysis revealed that expression of elongation factor (EF) 1 Bbeta and two ribosomal protein (RP) genes S7 and L7A was enhanced following exposure to 3 degrees C, but not during dehydration or exogenous ABA treatment. The mRNA levels of EF1Bbeta, RPS7, and RPL7A did not vary between cultivars with different frost tolerance, but differences in expression were found between different species. Experiments with an albino mutant and etiolated plants revealed that the cold-dependent regulation of EF1Bbeta, RPS7, and RPL7A transcripts is controlled by a chloroplast-related pathway impaired in the mutant
Keyword	[ Hide all but 1 of 41 ] aba aba treatment acclimation albino plant analysis arabidopsis barley clone cold cold acclimation component cultivar dehydration elongation elongation factor est exposure expression frost tolerance gene genotypes level library light low temperature mrna mutant northern northern analysis pathway protein protein synthesis regulation ribosomal protein solanum tuberosum l species synthesis temperature tolerance transcript wheat

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