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GrainGenes Reference Report: JPY-158-1189

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Reference	JPY-158-1189
Title	Molecular cloning and characterization of salt responsive genes in rice (Oryza sativa)
Journal	Journal of Plant Physiology
Year	2001
Volume	158
Pages	1189-1194
Author	Pillai MA Yanagihara S Akiyama T
Abstract	Summary: Differential screening was performed on a cDNA library made from RNAs isolated from rice seedlings (variety TSA2), grown in water culture with 3 h osmotic shock (150 mmol/L NaCl). Sixteen cDNA clones showing preferential hybridization to RNA from salt shocked seedlings were isolated. Three clones encoding salt responsive protein were selected by Northern hybridization study. One of the clones OSSRI represented a gene that is expressed at high levels during 24 h NaCl treatment. The DNA sequence analysis of OSSRI indicated a similarity to the deduced amino acid sequence of inositol phosphate kinase like protein of Arabidopsis thaliana. Another clone OSSRII showed identity to the photosystem II reaction center W protein of Arabidopsis thaliana. The clone OSSRIII was highly similar to cysteine proteinase inhibitor which is strongly associated with a chilling inducible and drought tolerant protein. Hybridization of the clone OSSRI to genomic DNA digested with EcoR1, EcoRV, Hind III, and Sa/1 indicated that a single copy of the gene encoded the salt inducible transcript. RNA blot hybridization showed that mRNA of 1.3, 1.0, 0.9 kb for the clones OSSRI, OSSRII and OSSRIII, respectively, accumulated over time up to 24 h
Keyword	[ Hide all but 1 of 53 ] amino acid amino acid sequence arabidopsis blot hybridization cdna cdna library chilling cloning complementary dna copies cysteine cysteine proteinase cysteine proteinase inhibitor differential screening dna dna hybridization drought gene genomic dna high-level hybridization inhibitor inositol inositol phosphate kinase kinase messenger rna molecular cloning mrna nacl northern northern hybridization nucleotide sequence oryza osmosis phosphate photosystem protein proteinase reaction center responsive genes rice rice seedling rnas salinity salt screening seedling sequence similarities sodium chloride stress transcript water

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