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GrainGenes Reference Report: JPY-158-1089

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Reference	JPY-158-1089
Title	Single cell analysis technique for comparison of specific mRNA abundance in plant cells
Journal	Journal of Plant Physiology
Year	2001
Volume	158
Pages	1089-1092
Author	Gallagher JA Koroleva OA Tomos DA Farrar JF Pollock CJ
Abstract	Summary: A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose: fructan fructosyltransferase) was demonstrated in mesophyll and parenchymatous bundle sheath cells. This method provides a reliable technique for the analysis of specific gene transcript abundance in individual plant cells
Keyword	[ Hide all but 1 of 27 ] actin barley leaves bundle-sheath bundle-sheath cells cdna libraries cell fructan fructosyltransferase gene gene expression hordeum hordeum vulgare invertase light lolium temulentum mesophyll method micro-sampling mrna pattern plant cells purification sampling single cell analysis sucrose transcript transcript abundance

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