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GrainGenes Reference Report: JPY-158-635

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Reference	JPY-158-635
Title	Expression of sucrose : fructan 6-fructosyltransferase (6-SFT) and myo-inositol 1-phosphate synthase (MIPS) genes in barley (Hordeum vulgare) leaves
Journal	Journal of Plant Physiology
Year	2001
Volume	158
Pages	635-643
Author	Wei JZ Chatterton NJ Larson SR
Abstract	Summary: Fructan is an important class of non-structural carbohydrates present in cool-season grasses. Sucrose:fructan 6-fructosyltransferase (6-SFT, EC 2.4.1.10), one of the enzymes thought to be involved in grass fructan biosynthesis, catalyzes the initiation and extension of 2,6-linked fructans. Myo-inositol is a central component in several metabolic pathways in higher plants. Myo-inositol 1-phosphate synthase (MIPS) (EC 5.5.1.4), the first enzyme in inositol de novo biosynthesis, catalyzes the formation of myo-inositol 1-phosphate (MIP) from glucose-6-phosphate. The expression of 6-SFT and MIPS genes is compared in barley (Hordeum vulgare L.) leaves under various conditions. In cool temperature treatments, both 6-SFT and MIPS mRNAs accumulate within two days and then decline after four days. Under warm temperatures and continuous illumination, the amount of 6-SFT and MIPS mRNA gradually accumulated in detached leaves and increased significantly by 8 h. In contrast, we observed no significant changes over time in attached (control) leaves. Treating detached leaves with glucose or sucrose in the dark resulted in accumulations of both 6-SFT and MIPS mRNA. Homologous expression patterns for 6-SFT and MIPS genes suggest that they may be similarly regulated in barley leaves. Although sucrose and glucose may play important roles in the expression of 6-SFT and MIPS genes, regulation likely involves multiple factors
Keyword	[ Hide all but 1 of 41 ] 6-fructosyltransferase arabidopsis thaliana barley barley leaves biosynthesis carbohydrate component dark enzyme expression patterns extension fructan fructan biosynthesis fructans gene gene expression gene regulation glucose higher-plants hordeum hordeum vulgare illumination induction inositol key enzyme metabolism mrna multiple myo-inositol myo-inositol 1-phosphate synthase (mips) pathway pattern regulation rice roles stress sucrose sucrose : fructan 6-fructosyltransferase (6-sft) synthase temperature tolerance

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