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GrainGenes Reference Report: JPY-158-91

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Reference	JPY-158-91
Title	Characterization of a cDNA encoding a type I starch branching enzyme produced in developing wheat (Triticum aestivum L.) kernels
Journal	Journal of Plant Physiology
Year	2001
Volume	158
Pages	91-100
Author	Repellin A Baga M Chibbar RN
Abstract	Summary: This report describes the characterization of a 2.8 kb full-length cDNA, coding for a starch branching enzyme I, denoted SBEI-2, which is produced in the wheat endosperm. The deduced SBEI-2 sequence predicted an 832 residue long precursor protein with a 61 amino acid long transit peptide at the amino terminus. A molecular mass of 87.4 kDa was calculated for the mature form of SBEI-2, which showed high sequence similarity (>82%) to SBEI characterized from rice and maize. All conserved amino acids at the catalytic site of amylolytic enzymes were preserved on SBEI-2, and the protein was active as a branching enzyme when produced in Escherichia coli. Analysis of SBEI gene expression during wheat kernel development showed that accumulation of 2.8 kb SBEI gene transcripts was highest around 15 days after anthesis
Keyword	[ Hide all but 1 of 38 ] a-type amino acid barley branching branching enzyme cdna characterization cloning conservation development endosperm enzyme expression analysis full length cdna gene gene expression iib kernel development kernels maize maize endosperm multiple forms peptide precursor residue rice rice seeds sequence sequence similarities site solanum tuberosum starch starch biosynthesis starch branching enzyme transcript transit peptide triticum aestivum wheat endosperm

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