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GrainGenes Reference Report: JPN-25-1063

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Reference	JPN-25-1063
Title	Inhibition of cell growth caused by aluminum toxicity results from aluminum-induced cell death in barley suspension cells
Journal	Journal of Plant Nutrition
Year	2002
Volume	25
Pages	1063-1073
Author	Pan JW Zhu MY Chen H Han N
Abstract	Summary: The inhibition of cell growth caused by aluminum (Al) toxicity was studied in barley (Hordeum vulgare L., cv. Zaoshu 3 from our laboratory) suspension cells in this paper. The growth of suspension cells in 0.1-5.0 mM Al treatment groups was obviously inhibited in 24-h Al treatments and was not recovered during 24-h recovery culture without Al. On the average, 46.70% or 83.36% of suspension cells lost viability respectively during 24-h Al treatments or 24-h recovery culture by the detection of trypan blue staining. The agarose gel electrophoresis shows that DNA ladders were induced after 24-h Al (0.1-5.0 mM) treatments. Al toxicity significantly inhibits cell division and promotes chromosomal variation, but the mitotic index (MI) of suspension cells is so low that the inhibition of Al toxicity on cell division does not cause the significant inhibition of cell growth after short-term exposure to Al. These results suggest that Al-induced inhibition of cell growth mainly results from cell death occurring during Al treatments or recovery culture, which can occur possibly via programmed cell death (PCD)-related signal transduction pathway
Keyword	[ Hide all but 1 of 48 ] al al toxicity al-toxicity aluminum aluminum toxicity barley cell cell death cell division cell growth cell-division cell-growth chromosomal variation culture death detection division dna dna ladder electrophoresis exposure gel electrophoresis gel-electrophoresis growth hordeum inhibit inhibition maize mechanism mi mitotic index paper pathway programmed cell death proton short term short-term signal signal transduction signal transduction pathway suspension suspension cells tolerance toxicity transduction transduction pathway trypan blue staining viability

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