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GrainGenes Reference Report: JPN-18-1225

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Reference	JPN-18-1225
Title	Field evaluation of the pedundle perfusion technique
Journal	Journal of Plant Nutrition
Year	1995
Volume	18
Pages	1225-1236
Author	Foroutan-pour K Ma B Smith D
Abstract	The peduncle perfusion system has been used successfully to evaluate plant responses to post-anthesis plant growth regulators (PGR) and nitrogen (N) applied through the peduncle under greenhouse conditions No experiment with this technique has been conducted in the field Because environmental conditions in the field and in the greenhouse are very different the utility of this technique in the field is still unknown The objectives of this study were to evaluate: i) the overall applicability of the peduncle perfusion technique in the field, and ii) the responses of barley grain protein concentration to grain-filling N and ethephon application through a peduncle perfusion system under field conditions The perfusion technique floods the peduncle interior with a selected solution for periods of weeks to months The plants were allowed to take up urea in one of two concentrations (15 and 30 millimole N) or ethephon, a PGR, at 15 micrometer from the peduncle for 29 days The total volume of solution taken up by the plants ranged from 35 to 81 mL. Peduncle perfusion with 30 millimole N (urea) increased spike protein concentration and content and grain protein concentration without affecting seed weight/spike. Peduncle perfusion with ethephon increased grain protein concentration, while spike protein content was not increased over distilled water perfused plants. Using the perfusion technique under field conditions showed that supplying physiology effectors to barley plants through the peduncle could be an appropriate method for studying nutrient translocation or effects of metabolism modifying substances in the field during the grain filling period.
Keyword	[ Hide all but 1 of 11 ] ethephon field-experimentation hordeum vulgare nutrient-uptake perfusion protein content seed seed-weight spikes stem urea

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