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GrainGenes Reference Report: JMT-59-141

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Reference	JMT-59-141
Title	Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan Probe
Journal	Journal of Microbiological Methods
Year	2004
Volume	59
Pages	141-146
Author	Reischer GH Lemmens M Farnleitner A Adler A Mach RL
Abstract	A new real-time PCR based method was developed for the species-specific detection, identification and quantification of Fusarium graminearum in planta . It utilizes a TaqMan hybridization probe targeting the beta-tubulin gene and a plasmid standard. The assay is highly specific giving no product with DNA of closely related species. It is very sensitive, detecting down to five gene copies per reaction, and is able to produce reliable quantitative data over a range of six orders of magnitude
External Databases	Pubmed: 15325762
Keyword	[ Hide all but 1 of 9 ] dna fungal diseases hybridization plant diseases plant pathogenic fungi plant pathogens polymerase chain reaction quantitative analysis quantitative techniques

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