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GrainGenes Reference Report: JGR-12-460

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Reference	JGR-12-460
Title	Improving biocontrol effect of Pseudomonas fluorescens P5 on plant diseases through genetic modification with chitinase gene
Journal	Journal of Agricultural Biotechnology
Year	2004
Volume	12
Pages	460-463
Author	Xu X Zhang L Zhu Y Tang W
Language	Chinese
Abstract	A study was conducted to improve the biological control efficiency of P. fluorescens strain P5 via the introduction of a chitinase gene. A 6.5 kb DNA fragment containing a chitinase gene was inserted in vector pDSK519 to construct a new plasmid, pDSK51965. The new plasmid was introduced in strain P5, forming a new strain, P5-1. Restriction digestion and chitin plate culture confirmed that P5-1 contained a functional chitinase gene. P5-1 had higher effect than P5 against rice sheath blight and cotton damping off cause by Rhizoctonia solani . P5-1 also had higher effect compared to P5 in controlling wheat take-all disease ( Gaeumannomyces graminis var. tritici )
Keyword	[ Hide all but 1 of 16 ] biological control biological control agents chitinase control cotton dna fungal antagonists gene expression genetically engineered microorganisms modification plant disease control plant diseases plant pathogenic fungi plant pathogens plasmid vectors rice

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