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GrainGenes Reference Report: JFC-51-5803

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Reference	JFC-51-5803
Title	Trypsin/alpha-amylase inhibitors inactivate the endogenous barley/malt serine endoproteinase SEP-1
Journal	Journal of Agricultural and Food Chemistry
Year	2003
Volume	51
Pages	5803-5814
Author	Jones BL Fontanini D
Abstract	Summary: Barley (Hordeum vulgare L.) malt contains endoproteinases belonging to all four of the commonly occurring classes, including serine proteinases. It also contains low molecular weight proteins that inhibit the activities of many of these endoproteinases, but it had never been shown that any barley or malt serine proteinases could be inhibited by any of these endogenous proteins. It is now reported that some proteins that were concentrated using an 'affinity' method inhibited the activity of a malt serine endoproteinase. Two-dimensional electrophoretic and in vitro analyses showed that the inhibited enzyme was serine endoproteinase 1 (SEP-1) and that the inhibition could be quantified using a semipurified preparation of this enzyme. Amino acid sequencing and MALDI-TOF MS were used to identify the components of the partially purified inhibiting fractions. Only the 'trypsin/(alpha)-amylase inhibitors' or chloroform/methanol (CM) proteins, most of which had truncated N and C termini, and one fragment of (beta)-amylase were present in the inhibitory fractions. When a CM protein fraction was prepared from barley according to traditional methods, some of its component proteins inhibited the activity of SEP-1 and some did not. This is the first report of the purification and identification of barley malt proteins that can inhibit an endogenous serine proteinase. It shows that some of the CM proteins probably play a role in controlling the activity of barley proteinases during germination, as well as possibly protecting the seed and young plant from microbes or pests
External Databases	Pubmed: 12952437
Keyword	[ Hide all but 1 of 31 ] bakers asthma disease barley beta amylase beta-amylase cloning cm proteins cv morex endoproteinase enzyme germination green malt endoproteinases hordeum hordeum vulgare in vitro inhibition insect alpha-amylase malt methods microbes pest protein protein fraction purification seed sep-1 sequencing serine serine proteinases tetrameric inhibitor vitro washington

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