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GrainGenes Reference Report: JFC-49-4903

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Reference	JFC-49-4903
Title	Study of metallopeptidase isozymes from malted barley (Hordeum vulgare cv. Morex)
Journal	Journal of Agricultural and Food Chemistry
Year	2001
Volume	49
Pages	4903-4911
Author	Fontanini D Jones BL
Abstract	Summary: It has been reported that germinated barley contains peptidases that are sensitive to metal-chelating agents; however, none of these enzymes have been isolated, nor have their roles in germinated barley been investigated. Anion-exchange chromatography and chromatofocusing have been used to isolate a group of peptidases from barley (Hordeum vulgare cv. Morex) green malt that are sensitive to metal-chelating agents. Their activities were studied using one- and two-dimensional polyacrylamide gel electrophoresis. When analyzed on two-dimensional PAGE gels that contained gelatin as substrate, the enzymes separated into three major and approximately six minor activity spots with acidic pI values. The enzymes were optimally active against the gelatin substrate at pH 8.0 and were completely inhibited by 1,10-phenanthroline and EDTA, indicating that they belonged to the metallopeptidase class (EC 3.4.24.x). After the enzymes were inhibited with EDTA, the activities were recovered in the presence of low concentrations of metal ions. The hydrolysis of gelatin substrate was also impaired by the presence of reducing agents. The metallopeptidases readily digested, in vitro, the barley prolamine D hordein, indicating that they may be involved in degrading storage proteins during barley germination
External Databases	Pubmed: 11600042
Keyword	[ Hide all but 1 of 48 ] anion exchange anion exchange chromatography barley chromatography cysteine endopeptidase edta electrophoresis endoproteolytic enzymes enzyme gel electrophoresis gels germinated barley germination green malt hordein hordeum hordeum vulgare hydrolysis in vitro ion isolate isozyme malt malted barley malting metal ion metallopeptidase nor page peptidases ph polyacrylamide polyacrylamide gel electrophoresis protease protein protein hydrolysis proteinase proteolytic activities purification roles seed sodium dodecyl sulfate spot storage storage protein substrate two-dimensional polyacrylamide gel electrophoresis washington

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