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GrainGenes Reference Report: JFC-49-359

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Reference	JFC-49-359
Title	Structure and dynamics of high molecular weight glutenin subunits of durum wheat (Triticum durum) in water and alcohol solutions studied by electron paramagnetic resonance and circular dichroism spectroscopies
Journal	Journal of Agricultural and Food Chemistry
Year	2001
Volume	49
Pages	359-365
Author	Calucci L Pinzino C
Abstract	Summary: Complementary information on the structure and dynamics of high molecular weight glutenin subunits (HMW-GS) of durum wheat (Triticum durum) was obtained by means of two spectroscopic techniques. Electron paramagnetic resonance was used to investigate the dynamics of the HMW-GS hydrated with two 2-propanol/water mixtures at temperatures between 268 and 308 K by specific spin labeling of their cysteine residues. Spectra were of a composite type, resulting from two populations of spin labels differing in molecular mobility, both undergoing isotropic rotational diffusion. Diffusional coefficients and populations of the fast- and slow-moving spin labels, determined by an accurate spectral line shape analysis, are discussed as a function of temperature and water content in the solvent systems. Far-UV circular dichroism was employed to provide information on the secondary structure of the HMW-GS in three different solvents [aqueous 50% (v/v) 2-propanol, aqueous 0.1% (v/v) trifluoroacetic acid, and trifluoroethanol]. For the first one, the influence of temperature on HMW-GS structure was also investigated
External Databases	Pubmed: 11170599
Keyword	[ Hide all but 1 of 37 ] breadmaking quality circular dichroism conformation cysteine cysteine residues durum durum wheat dynamics elasticity far-uv cd gluten glutenin glutenin subunit high molecular weight hmw glutenin subunits hmw-gs mixture mobility molecular weight population protein structure residue residues secondary structure shape analysis spectroscopy spectrum spin labeling epr structure subunit temperature triticum durum washington water water-content weight weight glutenin subunits

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