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GrainGenes Reference Report: JFC-47-5273

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Reference	JFC-47-5273
Title	Identification of alleles for complex gene loci Glu-A1,Glu-B1, and Glu-D1, which code for high molecular weight subunits of glutenin in Japanese hexaploid wheat varieties
Journal	Journal of Agricultural and Food Chemistry
Year	1999
Volume	47
Pages	5273-5277
Author	Nakamura H
Abstract	Summary: Seed storage proteins of Japanese wheat (Triticum aestivum) varieties were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to identify the alleles for complex gene loci, GluA1, Glu-B1, and Glu-D1, which code for high molecular weight (HMW) subunits of glutenin in Japanese hexaploid wheat varieties. These were identified by comparison of subunit mobility with those previously found in hexaploid wheat. Twenty-four different, major glutenin HMW subunits were identified, and each variety contained three to five subunits. Seventeen different glutenin subunit patterns were observed for 14 alleles in Japanese varieties. A catalog of alleles for the complex gene loci, Glu -A1, Glu-B1, and Glu-D1, that code for HMW subunits of glutenin in hexaploid wheat was compiled. Japanese varieties showed some special allelic variation in glutenin HMW subunits that was different from those in hexaploid wheats of other countries
Keyword	[ Hide all but 1 of 36 ] alleles allelic variation analogs and derivatives catalog of alleles complex loci electrophoresis electrophoresis,polyacrylamide gel gel-electrophoresis gene genetic variation gluten glutenin glutenin subunit hexaploid hexaploid wheat hexaploid wheats hmw hmw subunits of glutenin human japan loci mobility molecular weight molecular weight subunits sds page seed seed storage protein sodium sodium dodecyl sulfate polyacrylamide gel storage storage protein triticum triticum aestivum weight weight subunits wheat varieties

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