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GrainGenes Reference Report: JFC-47-5261

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Reference	JFC-47-5261
Title	Real-time quantitative PCR detection of genetically modified maximizer maize and roundup ready soybean in some representative foods
Journal	Journal of Agricultural and Food Chemistry
Year	1999
Volume	47
Pages	5261-5266
Author	Vatlingom M Pijnenburg H Gendre F Brignon P Vaitilingom M
Abstract	Summary: A fast and quantitative method was developed to detect transgenic 'Maximizer' maize 'event 176' (Novartis) and 'Roundup Ready' soybean (Monsanto) in food by real-time quantitative PCR. The use of the ABI Prism 7700 sequence detection system allowed the determination of the amplified product accumulation through a fluorogenic probe (TaqMan). Fluorescent dyes were chosen in such a way as to coamplify total and transgenic DNA in the same tube. Using real-time quantitative PCR, 2 pg of transgenic or total DNA per gram of starting sample was detected in 3 h after DNA extraction and the relative amounts of 'Maximizer' maize and 'Roundup Ready' soybean in some representative food products were quantified
Keyword	[ Hide all but 1 of 29 ] alkyl (aryl) transferases bacillus thuringiensis bacterial toxins cereal products crystal proteins detection dna dna extraction dna probes extraction food foods glycine max gmo lectins maize maize starch pcr phosphatidylcholines polymerase chain reaction products quantitative pcr sequence soy protein soybean transgenic transgenic plant zea mays zein

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