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GrainGenes Reference Report: JEB-54-1101

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Reference	JEB-54-1101
Title	Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum
Journal	Journal of Experimental Botany
Year	2003
Volume	54
Pages	1101-1111
Author	Anand A [ Show all 6 ]
Abstract	Summary: Genes encoding pathogenesis-related (PR-) proteins isolated from a cDNA library of Fusarium graminearum-infected wheat spikes of scab-resistant cultivar 'Sumai-3' were transformed into susceptible spring wheat, 'Bobwhite' using a biolistic transformation protocol, with the goal of enhancing levels of resistance against scab. Twenty-four putative transgenic lines expressing either a single PR-protein gene or combinations thereof were regenerated. Transgene expression in a majority of these lines (20) was completely silenced in the T1 or T2 generations. Four transgenic wheat lines showed stable inheritance and expression of either a single transgene or transgene combinations up to four generations. One line co-expressing a chitinase and beta-1,3-glucanase gene combination, when bioassayed against scab showed a delay in the spread of the infection (type II resistance) under greenhouse conditions. This line and a second transgenic line expressing a rice thaumatin-like protein gene (tlp) which had moderate resistance to scab in previous greenhouse trials, along with susceptible and resistance checks were evaluated for resistance to scab under field conditions. None of the transgenic lines had resistance to scab in the field under conditions of strong pathogen, suggesting these plants lacked effective resistance to initial infection (type I resistance) under these conditions. As far as is known, this is the first report of field evaluation of transgenic wheat expressing genes for PR-proteins against disease resistance
External Databases	Pubmed: 12598580
Keyword	[ Hide all but 1 of 43 ] antifungal protein barley beta-1,3-glucanase cdna cdna library chitinase cultivar defense response genes disease field field evaluation fungal resistance fusarium fusarium graminearum gene genetic transformation graminearum infection inheritance mosaic virus pathogen pr protein pr proteins protocol resistance rice scab selectable marker spikes spring spring wheat thaumatin-like protein tobacco transformation transgene transgene expression transgene silencing transgenic transgenic wheat trials wheat lines wheat scab wheat spikes

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