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GrainGenes Reference Report: JEB-54-951

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Reference	JEB-54-951
Title	A cathepsin B-like cysteine protease gene from Hordeum vulgare (gene CatB) induced by GA in aleurone cells is under circadian control in leaves
Journal	Journal of Experimental Botany
Year	2003
Volume	54
Pages	951-959
Author	Martinez M Rubio-Somoza I Carbonero P Diaz I
Abstract	Summary: A barley cDNA clone encoding a putative cysteine protease with sequence homology to cathepsin B from mammalian cells has been characterized. This barley gene (CatB) is ubiquitously expressed, its mRNA being detected in leaves and roots, immature, mature and germinating embryos, in developing endosperms, and in aleurones upon germination, as assessed by northern blot analysis. The CatB mRNA expression in leaves increased by cold shock (6 degrees C), was not affected by wounding, and was under circadian control. These transcripts increased in the aleurone upon germination, whereas those for a cystatin encoding gene (Icy), that inhibits commercial cathepsin B in vitro, decreased. Gibberellin (GA) treatment of isolated barley aleurones induced and abscisic acid (ABA) repressed the steady-state levels of CatB mRNA, while Icy expression had an opposite pattern of mRNA accumulation in aleurones treated with GA. No response to GA or ABA was detected in leaves
External Databases	Pubmed: 12598566
Keyword	[ Hide all but 1 of 40 ] aba abiotic stress acid aleurone barley barley aleurone barley cathepsin-b expression barley gene barley-aleurone cdna cdna clone cdna cloning cell cereal aleurone circadian rhythm clone cold control cystatin cysteine encoding gene endosperm expression pattern gene germination germination hormonal regulation gibberellin homology hordeum hormonal regulation in vitro mammalian cells molecular cloning pattern protease proteinase rice seeds sequence transcript zea mays

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