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GrainGenes Reference Report: JEB-53-727

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Reference	JEB-53-727
Title	IDI7, a new iron-regulated ABC transporter from barley roots, localizes to the tonoplast
Journal	Journal of Experimental Botany
Year	2002
Volume	53
Pages	727-735
Author	Yamaguchi H Nishizawa NK Nakanishi H Mori S
Abstract	Summary: A new Fe-deficiency-induced cDNA, IDI7, was isolated from the roots of Fe-deficient barley (Hordeum vulgare L. cv. Ehimehadaka no. 1). The transcript levels of IDI7 in roots strongly correlated with iron nutritional status, and induction by Fe-deficiency was restricted to roots. Excess treatment with heavy metal ions, such as copper, manganese, and zinc, did not cause obvious IDI7 induction in either leaves or roots. IDI7 encodes a 644 amino acid protein, and has features typical of ATP-binding cassette (ABC) transporters. Phylogenetic analysis revealed that IDI7 is closely related to the half-type ABC protein subfamily, which includes mammalian transporters associated with antigen processing (TAPs). A transiently expressed fusion protein of IDI7 to green fluorescent protein (GFP) was localized to tonoplasts in suspension-cultured tobacco (Nicotiana tabacum L.) cells. IDI7 and its orthologues are thought to comprise a new class of ABC transporters, located in the tonoplasts of higher plants. A possible Fe-deficiency adaptation role for IDI7 in barley root cells, involving transport across the tonoplast, is proposed
External Databases	Pubmed: 11886893
Keyword	[ Hide all but 1 of 57 ] abc protein abc transporter acid adaptation amino acid antigen arabidopsis thaliana atp-binding barley barley roots bean-plants cdna cell class-ii region copper deficiency stress encode fe deficiency fe-deficiency features fusion fusion protein gene gfp green fluorescent protein higher plant hordeum hordeum vulgare induction ion iron localizes manganese metal ion metal-binding peptides metal-ions nicotiana nicotiana tabacum nutritional status phylogenetic analysis processing protein protein subfamily rhizosphere acidification root root cells subfamilies tabacum tobacco tonoplast transcript transcript levels transport transporter vacuole yeast zinc

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