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GrainGenes Reference Report: JEB-53-147

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Reference	JEB-53-147
Title	PM19, a barley (Hordeum vulgare L.) gene encoding a putative plasma membrane protein, is expressed during embryo development and dormancy
Journal	Journal of Experimental Botany
Year	2002
Volume	53
Pages	147-148
Author	Ranford JC Bryce JH Morris PC
Abstract	Summary: A barley (Hordeum vulgare L.) cDNA, PM19, encoding a putative plasma membrane protein was isolated through differential screening of a dormant wild oat embryo library. PM19 is expressed in barley embryos from mid-embryogenesis up to maturity. PM19 mRNA levels decline upon germination, whereas dormant embryos retained high levels of message for up to 72 h of imbibition. PM19 mRNA levels also remained high or were reinduced in non-dormant embryos by treatments that prevented germination (250 mm NaCl, 10% sorbitol, or 50 micrometer ABA). The PM19 protein sequence is highly conserved in monocotyledonous and dicotyledonous plants
External Databases	Pubmed: 11741051
Keyword	[ Hide all but 1 of 36 ] aba acid barley barley embryo cdna development dicotyledonous plants differential screening dormancy embryo embryo development embryos freezing tolerance gene germination high-level hordeum hordeum vulgare imbibition level library maturity membrane membrane protein mrna nacl oat plasma membrane protein screening seed dormancy sequence sorbitol stress vulgare wild

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