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GrainGenes Reference Report: JEB-52-2387

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Reference	JEB-52-2387
Title	Expression patterns of genes encoding endomembrane proteins support a reduced function of the Golgi in wheat endosperm during the onset of storage protein deposition
Journal	Journal of Experimental Botany
Year	2001
Volume	52
Pages	2387-2388
Author	Shy G Ehler L Herman E Galili G
Abstract	Summary: Wheat storage proteins are deposited in the vacuole of maturing endosperm cells by a novel pathway that is the result of protein body formation by the endoplasmic reticulum followed by autophagy into the central vacuole, bypassing the Golgi apparatus. This model predicts a reduced role of the Golgi in storage protein accumulation, which has been supported by electron microscopy observations. To study this issue further, wheat cDNAs encoding three distinct proteins of the endomembrane system were cloned and characterized. The proteins encoded were homologues (i) of the ER translocon component Sec61alpha, (ii) the vacuolar sorting receptor BP-80 which is located in the Golgi and clathrin-coated prevacuole vesicles (CCV), and (iii) the Golgi COPI coatomer component COPalpha. During endosperm development, the levels of all three mRNAs were highest in young stages, before the onset of storage protein synthesis, and declined with seed maturation. However, the relative mRNA levels of BP-80/Sec61alpha and the COPalpha/Sec61alpha were lower during the onset of storage protein synthesis than at earlier stages of endosperm development. These results support previous studies, suggesting a reduced function of the Golgi apparatus in wheat storage protein transport and deposition
External Databases	Pubmed: 11709589
Keyword	[ Hide all but 1 of 46 ] alpha apparatus bodies body formation cdna cell component deposition electron microscopy endomembrane system endoplasmic reticulum endosperm endosperm development er expression patterns function gene golgi golgi apparatus maturation microscopy model mrna onset pathway pattern protein protein accumulation protein bodies protein body protein synthesis protein transport receptor seed seed maturation sorting storage storage protein storage vacuoles synthesis system transport vacuole vesicles wheat endosperm wheat storage proteins

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