Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: JEB-52-1209

[Submit comment/correction]

Reference	JEB-52-1209
Title	Mapping of QTLs associated with cytosolic glutamine synthetase and NADH-glutamate synthase in rice (Oryza sativa L.)
Journal	Journal of Experimental Botany
Year	2001
Volume	52
Pages	1209-1217
Author	Obara M [ Show all 7 ]
Abstract	Ninety-eight backcross inbred lines (BC1F6) developed between Nipponbare, a japonica rice, and Kasalath, an indica rice were employed to detect putative quantitative trait loci (QTLs) associated with the contents of cytosolic glutamine synthetase (GS1; EC 6.3.1.2) and NADH-glutamate synthase (NADH-GOGAT; EC 1.4.1.14) in leaves. Immunoblotting analyses showed transgressive segregations toward lower or greater contents of these enzyme proteins in these backcross inbred lines. Seven chromosomal QTL regions for GS1 protein content and six for NADH-GOGAT protein content were detected. Some of these QTLs were located in QTL regions for various biochemical and physiological traits affected by nitrogen recycling. These findings suggested that the variation in GS1 and NADH-GOGAT protein contents in this population is related to the changes in the rate of nitrogen recycling from senescing organs to developing organs, leading to changes in these physiological traits. Furthermore, a structural gene for GS1 was mapped between two RFLP markers, C560 and C1408, on chromosome 2 and co-located in the QTL region for one-spikelet weight. A QTL region for NADH-GOGAT protein content was detected at the position mapped for the NADH-GOGAT structural gene on chromosome 1. A QTL region for soluble protein content in developing leaves was also detected in this region. Although fine mapping is required to identify individual genes in the future, QTL analysis could be a useful post-genomic tool to study the gene functions for regulation of nitrogen recycling in rice
External Databases	Pubmed: 11432939
Keyword	[ Hide all but 1 of 49 ] backcross cellular-localization chromosome cytosolic glutamine synthetase enzyme fine mapping function gene glutamine glutamine synthetase gs1 immunoblotting inbred lines indica indica rice japonica leaf blades line loci mapping marker molecular dissection nadh-glutamate synthase nitrogen nitrogen recycling oryza oryza sativa population position protein protein content qtl qtl analysis quantitative trait quantitative trait loci rate region regulation rflp rflp marker rice segregation structural gene synthase synthetase tool trait vascular bundles weight

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.