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GrainGenes Reference Report: JEB-52-857

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Reference	JEB-52-857
Title	Age-dependent transformation frequency in elite wheat varieties
Journal	Journal of Experimental Botany
Year	2001
Volume	52
Pages	857-863
Author	Pastori GM [ Show all 6 ]
Abstract	Summary: Wheat is a major world crop and as such is a primary target for improvement of agronomic characteristics via genetic engineering. Optimization of transformation is essential in order to overcome the relatively low transformation frequencies encountered with wheat. Transformation of elite wheat varieties is not always successful due to variability in regeneration and transformation frequencies between varieties. In this work, two elite wheat varieties with a relatively high embryogenic capacity were transformed by particle bombardment. A strong correlation between transformation frequency and the age of wheat donor plants was observed in both varieties. The mean transformation frequency rose from 0.7% to 5% when using immature embryos from old and young donor plants, respectively. This was observed in both varieties, the best bombardments achieving up to 7.3% frequency. Using explants at an optimal developmental stage from donor plants grown under environmentally-controlled conditions has improved the reproducibility of transformation efficiency of elite wheat varieties and leads to the production of apparently phenotypically normal, fertile, transgenic plants
External Databases	Pubmed: 11413223
Keyword	[ Hide all but 1 of 42 ] age agronomic characteristics bar gene bombardment callus capacity correlation crop elite wheal varieties embryo explants fertile fertile transgenic wheat frequencies genetic genetic engineering genetic transformation immature embryo improvement lead microprojectile bombardment old optimization particle bombardment plant-regeneration plant-science production regeneration reproducibility scutellar tissues target transformation transformation frequency transgenic transgenic plant transgenic plants triticum aestivum uida gene variability wheat transformation wheat varieties world

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