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GrainGenes Reference Report: JEB-51-1179

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Reference	JEB-51-1179
Title	Induced activity of adenine phosphoribosyltransferase (APRT) in iron-deficient barley roots: a possible role for phytosiderophore production
Journal	Journal of Experimental Botany
Year	2000
Volume	51
Pages	1179-1188
Author	Itai R [ Show all 7 ]
Abstract	Summary: To isolate the genes involved in the response of graminaceous plants to Fe-deficient stress, a protein induced by Fe-deficiency treatment was isolated from barley (Hordeum vulgare L.) roots. Based on the partial amino acid sequence of this protein, a cDNA (HvAPT1) encoding adenine phosphoribosyltransferase (APRT:EC 2.4.2.7) was cloned from a cDNA library prepared from Fe-deficient barley roots. Southern analysis suggested that there were at least two genes encoding APRT in barley. Fe deficiency increased HvAPT1 expression in barley roots and resupplying Fe to the Fe-deficient plants rapidly negated the increase in HvAPT1 mRNA. Analysis of localization of HvAPT1-sGFP fusion proteins in tobacco BY-2 cells indicated that the protein from HvAPT1 was localized in the cytoplasm of cells. Consistent with the results of Northern analysis, the enzymatic activity of APRT in barley roots was remarkably increased by Fe deficiency. This induction of APRT activity by Fe deficiency was also observed in roots of other graminaceous plants such as rye, maize, and rice. In contrast, the induction was not observed to occur in the roots of a non-graminaceous plant, tobacco. Graminaceous plants generally synthesize the mugineic acid family phytosiderophores (MAs) in roots under Fe-deficient conditions. In this paper, a possible role of HvAPT1 in the biosynthesis of MAs related to adenine salvage in the methionine cycle is discussed
External Databases	Pubmed: 10937693
Keyword	[ Hide all but 1 of 33 ] amino acid amino acid sequence aprt arabidopsis thaliana barley barley roots biosynthesis cdna cytoplasm deficiency enzyme family fe deficiency gene hordeum vulgare induction library localization maize metabolism methionine methionine cycle mrna mugineic acid nicotiana ine phytosiderophore rice root rye sequence stress tobacco tobacco by-2 cells

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