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GrainGenes Reference Report: JEB-50-311

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Reference	JEB-50-311
Title	Reduced aleurone alpha-amylase production in aged wheat seeds is accompanied by lower levels of high-pl alpha-amylase transcripts and reduced response to gibberellic acid.
Journal	Journal of Experimental Botany
Year	1999
Volume	50
Pages	311-317
Author	Bernal-Lugo I Rodriguez M Gavilanes-Ruiz M Hamabata A
Abstract	With the aim of determining the level at which ageing exerts its effect on the expression of alpha-amylase, GA3 regulation of alpha-amylase production was studied in isolated aleurone layers from aged wheat seeds. GA3-induced a-amylase activity was lower in the tissue from aged seeds than in controls. However, the proportion of 35S-methionine incorporated into alpha-amylase was higher in the aged than in control tissue. The pattern of alpha-amylase isoforms was resolved by isoelectric focusing and showed that two isogroups were present with the activity of the high-pl isogroup being higher in the control than in the aged lot. These apparently contradictory results may be explained in terms of differences in isozyme expression. Studies on the expression of alpha-amylase genes indicated a reduction in the level of high-Pl mRNA in aged tissue. Dose-response curves showed lower GA3-responsiveness of aleurone layers from aged seeds as compared to the controls. From these results, it is proposed that the diminished capacity of alpha-amylase production in aleurone from aged seeds is apparently due to a decrease in the expression of the high-pl alpha-amylase genes, and this reduction is associated with a decrease in the response to GA3.
External Databases	Agricola: 2011-27060
Keyword	[ Hide all but 1 of 12 ] aging aleurone layer alpha amylase biosynthesis enzyme activity gene expression gibberellic acid isoelectric point isoenzyme messenger rna regulation triticum aestivum

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