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GrainGenes Reference Report: JCS-34-285

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Reference	JCS-34-285
Title	Relationships between barley hordeins and malting quality in a mutant of cv. triumph I. Genotype by environment interaction of hordein content
Journal	Journal of Cereal Science
Year	2001
Volume	34
Pages	285-294
Author	Molina-Cano JL [ Show all 6 ]
Abstract	Summary: The genetic (G), environmental (E) and G x E effects on total grain protein and hordein fractions, have been studied in a mutant (TL43) and its parent cultivar, the malting barley Triumph, in various seasons at Dundee (E Scotland) and Lleida (NE Spain). The grain protein content of TL43 was consistently about 2% higher than that of Triumph across environments. Differences in hordein composition were shown by SDS-PAGE electrophoresis. TL-43 had three B-hordein bands not present in Triumph which had a further three bands of the same hordein group not present in TL43. TL43 showed higher B-hordein content than Triumph in Scotland but lower in Spain, whereas the mutant showed consistently higher C- and D-hordein content than Triumph across environments, i.e. there was crossover G x E interaction for B-hordein and non-crossover for C- and D-hordein content. There were also differences in grain ultra-structure between the two lines, as TL43 showed a more dense protein matrix than Triumph, together with thinner pericarp, testa and aleurone layers. It was concluded that the mutation in TL43 had a significant effect on storage protein composition
Keyword	[ Hide all but 1 of 47 ] aleurone aleurone layer barley barley mutants composition cultivar different european regions electrophoresis environment environment interaction environmental extract development food fraction g x e interaction genotype genotype by environment interaction grain development grain protein grain protein content grain ultra-structure hordein hordein content hordeins layer layers malting malting barley malting quality matrix mechanism mutant mutation pericarp protein protein accumulation protein composition protein content quality relationships scotland sds page spain storage storage protein ultrastructure yield

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