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GrainGenes Reference Report: JCS-34-159

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Reference	JCS-34-159
Title	Isolation and characterisation of cDNAs encoding protein disulphide isomerases and cyclophilins in wheat
Journal	Journal of Cereal Science
Year	2001
Volume	34
Pages	159-171
Author	Johnson JC Clarke BC Bhave M
Abstract	The enzyme families of protein disulphide isomerases (PDI) and cyclophilins catalyse the isomerisation of disulphide bonds and the rotation of Xaa-Pro peptide bonds in nascent proteins, respectively, making them excellent candidates for regulating the deposition of storage proteins in wheat. This study aimed to isolate and characterise clones encoding cyclophilins and PDIs from a wheat endosperm cDNA library. A number of cDNA clones were isolated, of which three different cDNAs each of PDI and cyclophilin were selected for complete sequencing. The three PDI cDNAs encoded putative protein products of 513 or 516 amino acids, all exhibiting conserved sequences for the N-terminal signal peptide, the two thioredoxin-like domains at the catalytic sites and the C-terminal ER retention signal. The three cyclophilin cDNAs exhibited 68-87% identity to other reported cyclophilins and encoded putative protein products of 171 amino acids containing the conserved tryptophan residue and a 7 amino acid sequence unique to certain plant cyclophilins. The sequence variations within and outside the coding regions of all the cDNAs suggest that both enzymes are encoded by multiple genes. This information will allow further investigations into the roles of these enzymes in storage protein deposition in the developing endosperm and thus in wheat quality
Keyword	[ Hide all but 1 of 52 ] acid amino acid amino acid sequence beta-subunit binding-protein bonds cdna cdna clone cdna libraries cis-trans-isomerase clone cyclophilin a deposition disulfide-isomerase domain endosperm enzyme er escherichia coli family food gene gene sequence isolate isolation isomerases library messenger rna molecular-cloning multiple multiple genes nucleotide sequence peptide peptidyl prolyl cis-trans isomerase products prolyl 4-hydroxylase protein protein disulphide isomerase quality residue retention rotation sequence sequence variation sequencing signal signal peptide site storage storage protein wheat endosperm wheat quality

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