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GrainGenes Reference Report: JCS-21-5

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Reference
JCS-21-5
Title
Genetic analysis of low Mr glutenin subunits fractionated by two-dimensional electrophoresis (A-PAGE x SDS-PAGE)
Journal
Journal of Cell Science
Year
1995
Volume
21
Pages
5-13
Author
Redaelli R
Morel M
Autran J
Pogna N
Abstract
Alkylated glutenin subunits of F7 progenies from the cross between the Italian bread wheat cultivar Costantino and the Canadian cultivar Neepawa were fractionated by one-dimensional A-PAGE and SDS-PAGE and by two-dimensional A PAGE X SDS-PAGE Each gliadin allele at the Gli-1 loci of the parental cultivars was shown to be associated with a specific allele at each of the Glu-3 loci, at which low Mr glutenin subunits are encoded The Glu-A3 locus was found to code for two low Mr subunits in Neepawa and three in Costantino In this latter cultivar, eight low Mr subunits were assigned to each of the Glu-B3 and Glu-D3 loci, whereas seven subunits were attributed to the GluB3 locus and seven to the Glu-D3 locus in Neepawa A-PAGE X SDS-PAGE can be employed for a detailed description of low Mr subunits of glutenin in different cultivars following a genetic approach based on the correspondence between the alleles at the Gli-1 and Glu-3 loci
Keyword
[ Hide all but 1 of 6 ]
breadmaking
electrophoresis
fractionation
genetic
glutenin
wheat

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