Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: JBC-266-1564

[Submit comment/correction]

Reference	JBC-266-1564
Title	Biochemical and molecular characterization of three barley seed proteins with antifungal properties
Journal	Journal of Biological Chemistry
Year	1991
Volume	266
Pages	1564-1573
Author	Leah R Tommerup H Svendsen I Mundy J
Type	Article
Language	English
Abstract	We have purified three proteins from barley (Hordeum vulgare L.) seeds which synergistically inhibit the growth of fungi measured in a microtiter well assay. The proteins are a 26-kDa chitinase, a 30- kDa ribosome-inactivating protein, and a 32-kDa (1-3)-.beta.- glucanase. Full-length cDNAs encoding them were isolated and sequenced to determine the complete primary structures of the proteins. Northern hybridizations with the cDNAs as probes showed that the corresponding mRNAs accumulate differentially during seed development and germination. Chitinase mRNA accumulates to high levels in aleurone cells during late seed development and early germination, while high levels of mRNA encoding the ribosome- inactivating protein accumulate only in the starchy endoserm during late seed development. The glucanase mRNA accumulates to low levels during seed development and to higher levels in aleurone and seedling tissues during germination. Southern hybridizations showed that the three proteins are encoded by small families of three to eight genes. Their biological roles and potential use in genetic engineering studies are discussed.
Probe	[ Hide all but 1 of 6 ] pcBGL32 cCHI26 cBGL32 cRIP30 cRIP30A pcRIP30

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.