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GrainGenes Reference Report: ISP-22-89

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Reference	ISP-22-89
Title	A modified mini-prep method for economical and rapid extraction of genomic DNA in plants
Journal	Plant Molecular Biology Reporter
Year	2004
Volume	22
Pages	89
Author	Khan IA Awan FS Ahmad A Khan AA
Abstract	Molecular markers for map-based cloning, marker-assisted selection in crop breeding, and genetic studies require DNA isolation from a large number of plants in a short span of time. Here we describe a modified DNA extraction method that is economical in terms of cost, time, and labour. The method allows DNA extraction from as little as 0.2-0.3 g of leaves that are homogenized in zipper plastic bags, followed by DNA isolation in 1.5-ml Eppendorf tubes. By using the modified method, a DNA yield of 700-800 micro g/300 mg leaf tissue was obtained from cotton and wheat samples. The quality of the DNA was quite suitable for PCR-based markers
Keyword	[ Hide all but 1 of 16 ] agriculture biotechnology chemical composition cotton dna dna cloning extraction genetic mapping genetic marker genome isolation methodology plant composition polymerase chain reaction quality selection

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