GrainGenes Reference Report: IFM-95-333
Reference
IFM-95-333
Title
Specific detection of Fusarium langsethiae and related species by DGGE and ARMS-PCR of a beta -tubulin (tub1) gene fragment
Journal
International Journal of Food Microbiology
Year
2004
Volume
95
Pages
333-339
Author
Mach RL
[ Show all 6 ]
Abstract
Fusarium langsethiae was recently described as a new toxigenic Fusarium species, which morphologically resembles Fusarium poae, but exhibits a mycotoxin pattern related to Fusarium sporotrichioides. To develop tools for early and specific detection of F. langsethiae and distinguishing it from related species of section Sporotrichiella and Discolor (F. poae, F. sporotrichioides, Fusarium kyushuense, Fusarium robustum, Fusarium sambucinum and Fusarium tumidum) sequence variations in their beta -tubulin-encoding (tub1) gene were employed to design two PCR-based methods, denaturing gradient gel electrophoresis (DGGE) and amplification refractory mutation system (ARMS)-PCR. DGGE reliably separated all these strains, even from mixtures and in the presence of DNA from their natural hosts Zea mais, Triticum aestivum and Avena sativa. In addition, a tetraprimer ARMS-PCR, which employs two primer pairs to amplify, respectively, two different fragments of tub1 in a single PCR reaction resulted in rapid differentiation between F. langsethiae, F. sporotrichioides and F. poae according to the number of amplicons (four, two and one, respectively). These two methods will thus be worthwhile tools in the specific detection of F. langsethiae in infected crops
External Databases
Pubmed: 15337597
Keyword
[ Hide all but 1 of 7 ] dna hosts new species nucleotide sequence phylogenetics taxonomy tubulin
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: IFM-95-333
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||