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GrainGenes Reference Report: HER-88-349

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Reference	HER-88-349
Title	Characterisation of mildew resistant wheat-rye substitution lines and identification of an inverted chromosome by fluorescent in situ hybridisation
Journal	Heredity
Year	2002
Volume	88
Pages	349-355
Author	Forsstrom PO Merker A Schwarzacher T
Abstract	Summary: Seven different mildew resistant wheat lines derived from crosses between triticale and bread wheat were examined by molecular cytogenetics and chromosome C-banding in order to determine their chromosomal composition. Genomic in situ hybridisation (GISH) showed the presence of rye germplasm in all the lines and identified three substitution lines, three double substitution lines and one addition-substitution line. C-banding identified rye chromosomes 1R and 4R in the addition-substitution line, rye chromosomes 1R and 6R in two substitution lines and 1R and 2R in the third line, and rye chromosome 1R in the three substitution lines. Two of the latter lines (7-102 and 7-169) contained a modified form of the chromosome; fluorescent in situ hybridisation (FISH) using five different repetitive DNA-probes showed a pericentric inversion of 1R in both lines. The breakpoints of the 1R inversion were between (1) the 5S rDNA site and the NOR-region on the satellite of the short arm, and (2) between two AAC(5) sites close to the centromere on the long arm. The role of the rye chromosomes in the mildew resistance, the utilisation of the inverted 1R and the significance of the lines in wheat breeding are discussed
External Databases	Pubmed: 11986870
Keyword	[ Hide all but 1 of 44 ] 5s 5s rdna bread breeding centromere chromosomal substitution composition cytogenetics dna probe dna sequence fish genome genomic in situ hybridisation germplasm gish group-5 hexaploid wheat in situ insitu hybridization inversion long arm mildew mildew resistance molecular cytogenetics nomenclature order organization pericentric inversion physical chromosome mapping rdna recombinants resistance resistant ribosomal rna genes rye rye chromosomes satellite site substitution substitution line translocation triticale triticum aestivum wheat breeding

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