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GrainGenes Reference Report: HER-84-46

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Reference	HER-84-46
Title	Chromosomal localization of cotransformed transgenes in the hexaploid cultivated oat Avena sativa L. using fluorescence in situ hybridization
Journal	Heredity
Year	2000
Volume	84
Pages	46-53
Author	Leggett JM Perret SJ Harper J Morris P
Abstract	Summary: Fluorescence in situ hybridization (FISH) was used to localize two transgenes (gus and bar), carried on plasmids pACT-1F and pUBA, respectively, on mitotic metaphase squashes of T1 plants of the cultivated hexaploid oat Avena sativa L. cotransformed by microprojectile bombardment of embryogenic callus. Among the eight progeny analysed by FISH in each of two lines, we detected plants null, hetero- and homozygous for the two genes in one line, and plants null and heterozygous for the two genes in the other line. Our results demonstrated that in the two independent transformation events, the gus and bar genes had inserted in the same position relative to each other. In each transformation event, the insertions occurred on D satellite (SAT) chromosomes bearing a C genome translocation
External Databases	Pubmed: 10692010
Keyword	[ Hide all but 1 of 48 ] avena avena sativa bar bar gene beta glucuronidase biolistics bombardment callus chromosome cotransformation dna dna hybridization embryogenic callus fish fluorescence fluorescence in situ hybridization gene gene location gene transfer genetic transformation genome gus hexaploid hexaploid oat hybridization in situ in situ hybridization insertion localization maize metaphase microprojectile microprojectile bombardment molecular mapping oat oat transformation physical mapping plasmids position progeny promoter recombination reporter gene transformation transgene transgenes transgenic plant translocation

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