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GrainGenes Reference Report: HER-83-249

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Reference
HER-83-249
Title
Simultaneous identification of A, B, D and R genomes by genomic in situ hybridization in wheat-rye derivatives
Journal
Heredity
Year
1999
Volume
83
Pages
249-252
Author
Sanchez-Moran E
Benavente E
Orellana J
Abstract
Summary: Multicolour genomic in situ hybridization was carried out in wheat-rye hybrids and in a wheat-rye translocation line. Different hybridization conditions and mixture compositions were used, and A, B and D genomes of hexaploid wheat as well as the R genome of rye were distinguished simultaneously in somatic cells. Combination of genomic and rDNA probes in multicolour in situ hybridization was also performed to identify chromosomes within a specific genome
Keyword
[ Hide all but 1 of 23 ]
aegilops
biotin
chemical reactions
chromosome
chromosome analysis
d genome
digoxigenin
dna hybridization
dna probes
fluorescence
genome
genome analysis
hexaploid wheat
hybridization
intergeneric hybridization
ribosomal dna
rye
secale cereale
translocation
translocation lines
triticale
triticum aestivum
triticum monococcum

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