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GrainGenes Reference Report: GIS-30-231

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Reference	GIS-30-231
Title	KNOX homeobox genes are sufficient in maintaining cultured cells in an undifferentiated state in rice
Journal	Genesis
Year	2001
Volume	30
Pages	231-238
Author	Ito Y Eiguchi M Kurata N
Abstract	Summary: We produced transgenic rice calli, which constitutively express each of four KNOX family class 1 homeobox genes of rice, OSH1, OSH16, OSH15, and OSH71, and found that constitutive and ectopic expression of such genes inhibits normal regeneration from transformed calli, which showed continuous growth around their shoot-regenerating stages. Transgenic calli transferred onto regeneration medium began to display green spots, a sign of regeneration, but most of the transformants continued to propagate green spots at given stages. In the normal shoot-regeneration process of calli, expression of endogenous OSH1 was restricted in presumptive shoot-regenerating regions of calli and not observed in other areas. This restricted expression pattern should be required for further differentiation of the regenerating shoots. Thus our present results support the proposed function that KNOX family class 1 homeobox genes play a role in the formation and maintenance of the undetermined meristematic state of cells
Keyword	[ Hide all but 1 of 34 ] apical meristem calli callus cell cultured-cells differentiation ectopic expression family formation function gene gene expression growth homeotic genes inhibit maintenance media oryza sativa pattern regeneration regenerative ability region rice shoot shoot meristem shoot regeneration spot state tissue culture transcription factors transformant transgenic transgenic plant transgenic rice

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