Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: GNM-46-330

[Submit comment/correction]

Reference	GNM-46-330
Title	The organization of genes tightly linked to the Ha locus in Aegilops tauschii, the D-genome donor to wheat
Journal	Genome
Year	2003
Volume	46
Pages	330-338
Author	Turnbull KM [ Show all 7 ]
Abstract	The grain hardness locus, Ha, is located at the distal end of the short arm of chromosome 5D in wheat. Three polypeptides, puroindoline-a, puroindoline-b, and grain softness protein (GSP-1), have been identified as components of friabilin, a biochemical marker for grain softness, and the genes for these polypeptides are known to be tightly linked to the Ha locus. However, this region of the chromosome 5D has not been well characterized and the physical distance between the markers is not known. Separate lambda clones containing the puroindoline-a gene and the puroindoline-b gene have been isolated from an Aegilops tauschii (the donor of the D genome to wheat) genomic lambda library and investigated. Considerable variation appears to exist in the organization of the region upstream of the gene for puroindoline-b among species closely related to wheat. Using in situ hybridization the genes for puroindoline-a, -b, and GSP-1 were demonstrated to be physically located at the tip of the short arm of chromosome 5 of A. tauschii. Four overlapping clones were isolated from a large-insert BAC library constructed from A. tauschii and of these one contained genes for all of puroindoline-a, puroindoline-b, and GSP-1. The gene for puroindoline-a is located between the other two genes at a distance no greater than approximately 30 kb from either gene. The BAC clone containing all three known genes was used to screen a cDNA library constructed from hexaploid wheat and cDNAs that could encode novel polypeptides were isolated.Key words: puroindolines, GSP-1, chromosome 5D, BAC library, tauschii.
External Databases	https://doi.org/10.1139/g02-124
Allele	Pinb-D1h (Triticum)
Keyword	[ Hide all but 1 of 38 ] aegilops bac library canada cdna cdna library chromosome chromosome 5d component d genome d-genome donor donor endosperm friabilin gene genome genomic grain hardness grain-softness protein gsp-1 ha locus hardness hardness locus hexaploid hexaploid wheat hybridization in situ in situ hybridization locus organization promoter protein puroindoline puroindoline-b region sequence analysis softness softness protein tauschii

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.