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GrainGenes Reference Report: GNM-45-1238

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Reference	GNM-45-1238
Title	Identification of wheat-barley translocations by sequential GISH and two-colour FISH in combination with the use of genetically mapped barley SSR markers
Journal	Genome
Year	2002
Volume	45
Pages	1238-1247
Author	Nagy ED Molnar-Lang M Linc G Lang L
Abstract	Summary: Five wheat-barley translocations in a wheat background were characterized through the combination of cytogenetic and molecular genetic approaches. The wheat chromosome segments involved in the translocations were identified using sequential GISH and two-colour FISH with the probes pSc119.2 and pAs1. The barley chromatin in these lines was identified using SSR markers. A total of 45 markers distributed over the total barley genome were selected from a recently published linkage map of barley and tested on the translocation lines. The following translocations were identified: 2DS.2DL-1HS, 3HS.3BL, 6BS.6BL-4HL, 4D-5HS, and 7DL.7DS-5HS. Wheat-barley disomic and ditelosomic addition lines for the chromosomes 3HS, 4H, 4HL, 5H, 5HL, and 6HS were used to determine the correct location of 21 markers and the position of the centromere. An intragenomic translocation breakpoint was detected on the short arm of the barley chromosome 5H with the help of SSR marker analysis. Physical mapping of the SSR markers on chromosomes 1H and 5H was carried out using the intragenomic and the interspecific translocation breakpoints, as well as the centromere, as physical landmarks
External Databases	Pubmed: 12502270
Keyword	[ Hide all but 1 of 25 ] addition line barley breakpoints canada centromere chromatin chromosome chromosome segments derivatives fish genome hybrid in situ hybridization insitu hybridization linkage linkage map map mapping microsatellite marker physical mapping sequential gish and fish ssr ssr marker translocation wheat-barley translocations

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